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Research article - Peer-reviewed, 2011

Cloning and functional characterization of genes involved in fatty acid biosynthesis in the novel oilseed crop Lepidium campestre L.

Eriksson, Dennis; Merker, Arnulf

Abstract

P>We aim at domesticating the cruciferous Lepidium campestre L. as a novel oilseed crop. The potential to modify the fatty acid profile of the seed oil to obtain a suitable industrial oil quality is being explored. Using degenerate primers, two cDNAs corresponding to a putative FATTY ACID ELONGASE 1 (FAE1) and a putative microsomal FATTY ACID DESATURASE 2 (FAD2), respectively, were obtained from a L. campestre RACE-ready cDNA library from developing fruits. The LcFAE1 cDNA contains a CDS (coding sequences) of 1521 nt and the LcFAD2 cDNA contains a CDS of 1152 nt. These L. campestre cDNAs both show high homology to other plant FAE1 and FAD2 genes. Functional complementation with the LcFAE1 and LcFAD2 in Arabidopsis thaliana fae1 and fad2 mutants, low in eicosenoic acid and linoleic acid, respectively, restores the wild-type fatty acid profile of the seed oil. Thus, development of L. campestre cultivars with a modified seed oil quality through up- or down-regulation of the cloned genes may be achieved provided that an efficient transformation protocol for this species is developed.

Keywords

L; campestre L; A; thaliana; FAE1; FAD2; functional complementation

Published in

Plant Breeding
2011, Volume: 130, number: 3, pages: 407-409
Publisher: WILEY-BLACKWELL

      SLU Authors

    • UKÄ Subject classification

      Agricultural Science
      Plant Biotechnology

      Publication identifier

      DOI: https://doi.org/10.1111/j.1439-0523.2010.01825.x

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/46577