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Review article2010Peer reviewed

Electrochemical impedance spectroscopy in label-free biosensor applications: multivariate data analysis for an objective interpretation

Lindholm-Sethson, Britta; Nyström, Josefina; Malmsten, Martin; Ringstad, Lovisa; Nelson, Andrew; Geladi, Paul


Electrochemical impedance spectroscopy plays an important role in biosensor science thanks to the possibility of finding specific information from processes with different kinetics at a chosen electrode potential in one experiment. In this paper we briefly discuss label-free impedimetric biosensors described in the literature. A novel method for neutral interpretation of impedance data is presented that includes complex number chemometrics. Three examples are given based on impedance measurements on synthetic biomembranes, in this case a lipid monolayer deposited on a mercury electrode. The interaction of various compounds with the monomolecular lipid layer is illustrated with the following: (1) different concentrations of magainin (Geladi et al. in Proc. Int. Fed. Med. Biomed. Eng. 9:219-220, 2005); (2) different derivatives of gramicidin A (Lindholm-Sethson et al. in Langmuir 24:5029-5032, 2007), and (3) an antimicrobial peptide (Ringstad et al. in Langmuir 24:208-216, 2008).


Impedance; Biosensor; Complex number chemometrics

Published in

Analytical and Bioanalytical Chemistry
2010, Volume: 398, number: 6, pages: 2341-2349

      UKÄ Subject classification

      Biochemistry and Molecular Biology
      Analytical Chemistry

      Publication identifier


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