Abstract

Electrochemical impedance spectroscopy plays an important role in biosensor science thanks to the possibility of finding specific information from processes with different kinetics at a chosen electrode potential in one experiment. In this paper we briefly discuss label-free impedimetric biosensors described in the literature. A novel method for neutral interpretation of impedance data is presented that includes complex number chemometrics. Three examples are given based on impedance measurements on synthetic biomembranes, in this case a lipid monolayer deposited on a mercury electrode. The interaction of various compounds with the monomolecular lipid layer is illustrated with the following: (1) different concentrations of magainin (Geladi et al. in Proc. Int. Fed. Med. Biomed. Eng. 9:219-220, 2005); (2) different derivatives of gramicidin A (Lindholm-Sethson et al. in Langmuir 24:5029-5032, 2007), and (3) an antimicrobial peptide (Ringstad et al. in Langmuir 24:208-216, 2008).

Keywords

Impedance; Biosensor; Complex number chemometrics

Published in

Analytical and Bioanalytical Chemistry
2010, Volume: 398, number: 6, pages: 2341-2349
Publisher: SPRINGER HEIDELBERG

SLU Authors

• Geladi, Paul

  • Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology
Analytical Chemistry


Publication identifier

DOI: https://doi.org/10.1007/s00216-010-4027-7

Permanent link to this page (URI)

https://res.slu.se/id/publ/47806