Uhlin, Ulla
- Department of Molecular Biology, Swedish University of Agricultural Sciences
Research article2004Peer reviewedOpen access
Uppsten M, Davis J, Rubin H, Uhlin U
Two nrdF genes of Mycobacterium tuberculosis code for different R2 subunits of the class Ib ribonucleotide reductase (RNR). The proteins are denoted R2F-1 and R2F-2 having 71% sequence identity. The R2F-2 subunit forms the biologically active RNR complex with the catalytic R1E-subunit. We present the structure of the reduced R2F-2 subunit to 2.2 Angstrom resolution. Comparison of the R2F-2 structure with a model of R2F-1 suggests that the important differences are located at the C-terminus. We found that within class Ib, the E-helix close to the iron diiron centre has two preferred conformations, which cannot be explained by the redox-state of the diiron centre. In the R2F-2 structure, we also could see a mobility of alphaE in between the two conformations. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved
FEBS Letters
2004, volume: 569, number: 1-3, pages: 117-122
Publisher: ELSEVIER SCIENCE BV
https://res.slu.se/id/publ/4799