Research article - Peer-reviewed, 2010
The Arabidopsis thaliana STYLISH1 Protein Acts as a Transcriptional Activator Regulating Auxin Biosynthesis
Eklund, Magnus; Ståldal, Veronika; Valsecchi, I; Cierlik, Izabela; Eriksson, Caitriona; Hiratsu, Keiichiro; Ohme-Takagi, Masaru; Sundström, Jens; Thelander, Mattias; Ezcurra, Ines; Sundberg, EvaAbstract
The establishment and maintenance of auxin maxima in vascular plants is regulated by auxin biosynthesis and polar intercellular auxin flow. The disruption of normal auxin biosynthesis in mouse-ear cress ( Arabidopsis thaliana) leads to severe abnormalities, suggesting that spatiotemporal regulation of auxin biosynthesis is fundamental for normal growth and development. We have shown previously that the induction of the SHORT-INTERNODES/STYLISH (SHI/STY) family member STY1 results in increased transcript levels of the YUCCA (YUC) family member YUC4 and also higher auxin levels and auxin biosynthesis rates in Arabidopsis seedlings. We have also shown previously that SHI/STY family members redundantly affect development of flowers and leaves. Here, we further examine the function of STY1 by analyzing its DNA and protein binding properties. Our results suggest that STY1, and most likely other SHI/STY members, are DNA binding transcriptional activators that target genes encoding proteins mediating auxin biosynthesis. This suggests that the SHI/STY family members are essential regulators of auxin-mediated leaf and flower development. Furthermore, the lack of a shoot apical meristem in seedlings carrying a fusion construct between STY1 and a repressor domain, SRDX, suggests that STY1, and other SHI/STY members, has a role in the formation and/or maintenance of the shoot apical meristem, possibly by regulating auxin levels in the embryo.Published in
Plant Cell2010, volume: 22, number: 2, pages: 349-363
Publisher: AMER SOC PLANT BIOLOGISTS
Authors' information
Eklund, Magnus
Swedish University of Agricultural Sciences, Department of Plant Biology
Ståldal, Veronika (Ståldal, Veronika)
Swedish University of Agricultural Sciences, Department of Plant Biology
Valsecchi, I
Swedish University of Agricultural Sciences, Department of Plant Biology
Cierlik, Izabela
Swedish University of Agricultural Sciences, Department of Plant Biology
Eriksson, Caitriona
Swedish University of Agricultural Sciences, Department of Plant Biology
Hiratsu, Keiichiro
Ohme-Takagi, Masaru
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Biology
Ezcurra, Ines
Swedish University of Agricultural Sciences, Department of Plant Biology
UKÄ Subject classification
Agricultural Science
Publication Identifiers
DOI: https://doi.org/10.1105/tpc.108.064816
URI (permanent link to this page)
https://res.slu.se/id/publ/48444