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Research article2001Peer reviewedOpen access

Factors affecting the survival of frozen thawed bovine in vitro produced blastocysts

Gustafsson, Hans; Larsson, Birgitta; Shamsuddin, M.; Jaakma, Ulle; Emanuelson, Ulf

Abstract

Factors Affecting the Survival of Frozen Thawed Bovine In Vitro Produced Blastocysts. The effect of some factors on the post-thaw survival of a total of 240 in vitro produced bovine blastocysts was investigated using logistic regression analysis. The explanatory variables tested were: type of culture medium before freezing (TCM 199 supplemented with BSA, BSAITS (BSA+insulin+transferrin+selenium), ECS (estrous cow serum) with or without BOEC (bovine oviductal epithelial cells), age of the blastocyst (Day 7, Day 8+9), morphological appearance before freezing (distinct=Q1 or indistinct=Q2 inner cell mass) and type of cryoprotectant (glycerol, 1.0 M or ethylene glycol, 1.6 M). The survival after thawing based on the post-thaw quality and the development after co-culture with BOEC for 24 and 48 hours. Day 7 blastocysts had an almost three times better chance of survival than Day 8+9 blastocysts. Q1, Day 8+9 blastocysts had higher odds to survive after 48 hours in culture than Q2 blastocysts (p<0.05). Blastocysts produced in BSAITS medium had the best chances of survival; however, the odds were not always significant. Blastocysts frozen in glycerol had a better post-thaw quality rating than those frozen in ethylene glycol; however, the difference in post-thaw,development at culture was not significant. The relationship between post-thaw quality and post-thaw development at culture was significant (p<0.05). The developmental stage and/or age of the embryo and culture medium where development up to blastocyst takes place affect the post-thaw survival of the bovine embryos.

Keywords

bovine; embryo age; insulin-transferrin-selenium; glycerol; ethylene glycol; post-thaw quality score

Published in

Asian-Australasian journal of animal sciences
2001, Volume: 14, number: 1, pages: 7-12
Publisher: ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC

      SLU Authors

    • Gustafsson, Hans

      • Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences

      • Larsson, Birgitta

        • Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences

        • UKÄ Subject classification

        Other Veterinary Science

        Publication identifier

        DOI: https://doi.org/10.5713/ajas.2001.7

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/48934