Eriksson, Jan
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Research article2013Peer reviewedOpen access
Detection and correction of interference in SRM analysis
Bao, Yifei; Waldemarson, Sofia; Zhang, Guoan; Wahlander, Asa; Ueberheide, Beatrix; Myung, Sunnie; Reed, Brian; Molloy, Kelly; Padovan, Julio; Eriksson, Jan; Neubert, Thomas A.; Chait, Brian T.; Fenyo, David
Abstract
Selected Reaction Monitoring (SRM) is a method of choice for accurate quantitation of low-abundance proteins in complex backgrounds. This strategy is, however, sensitive to interference from other components in the sample that have the same precursor and fragment masses as the monitored transitions. We present here an approach to detect interference by using the expected relative intensity of SRM transitions. We also designed an algorithm to automatically detect the linear range of calibration curves. These approaches were applied to the experimental data of Clinical Proteomic Tumor Analysis Consortium (CPTAC) Verification Work Group Study 7 and show that the corrected measurements provide more accurate quantitation than the uncorrected data. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
Keywords
Mass spectrometry; Selected reaction monitoring; Interference detection; Interference correction; Linear range detection
Published in
Methods
2013, Volume: 61, number: 3, pages: 299-303
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
SLU Authors
UKÄ Subject classification
Bioinformatics and Systems Biology
Analytical Chemistry
Publication identifier
DOI: https://doi.org/10.1016/j.ymeth.2013.05.008
Permanent link to this page (URI)
https://res.slu.se/id/publ/50297