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Research article - Peer-reviewed, 2013

Detection and correction of interference in SRM analysis

Bao, Yifei; Waldemarson, Sofia; Zhang, Guoan; Wahlander, Asa; Ueberheide, Beatrix; Myung, Sunnie; Reed, Brian; Molloy, Kelly; Padovan, Julio; Eriksson, Jan; Neubert, Thomas A.; Chait, Brian T.; Fenyo, David

Abstract

Selected Reaction Monitoring (SRM) is a method of choice for accurate quantitation of low-abundance proteins in complex backgrounds. This strategy is, however, sensitive to interference from other components in the sample that have the same precursor and fragment masses as the monitored transitions. We present here an approach to detect interference by using the expected relative intensity of SRM transitions. We also designed an algorithm to automatically detect the linear range of calibration curves. These approaches were applied to the experimental data of Clinical Proteomic Tumor Analysis Consortium (CPTAC) Verification Work Group Study 7 and show that the corrected measurements provide more accurate quantitation than the uncorrected data. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords

Mass spectrometry; Selected reaction monitoring; Interference detection; Interference correction; Linear range detection

Published in

Methods
2013, Volume: 61, number: 3, pages: 299-303
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

    UKÄ Subject classification

    Bioinformatics and Systems Biology
    Analytical Chemistry

    Publication identifier

    DOI: https://doi.org/10.1016/j.ymeth.2013.05.008

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/50297