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Research article2013Peer reviewedOpen access

The effect of substrate and operational parameters on the abundance of sulphate-reducing bacteria in industrial anaerobic biogas digesters

Moestedt, Jan; Nilsson Påledal, Sören; Schnürer, Anna

Abstract

This study evaluated the effects of operational parameters and type of substrate on the abundance of sulphate-reducing bacteria in 25 industrial biogas digesters using qPCR targeting the functional dissimilatory sulphite reductase gene. The aim was to find clues for operational strategies minimizing the production of H2S. The results showed that the operation, considering strategies evaluated, only had scarce effect on the abundance, varying between 10(5) and 10(7) gene copies per ml. However, high ammonia levels and increasing concentration of sulphate resulted in significantly lower and higher levels of sulphate-reducing bacteria, respectively. (C) 2013 Elsevier Ltd. All rights reserved.

Keywords

Biogas; Sulphate-reducing bacteria; Hydrogen sulphide; qPCR; dsrB

Published in

Bioresource Technology
2013, Volume: 132, pages: 327-332
Publisher: ELSEVIER SCI LTD