A novel selection system for potato transformation using a mutated AHAS gene

Abstract

Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a beta-glucuronidase (GUS) gene. Selection on 0.5 muM Imazamox yielded GUS expression in 93-100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.

Keywords

acetohydroxyacid synthase; AHAS; herbicide tolerance; selection gene; potato transformation

Published in

Plant Cell Reports
2003, volume: 22, number: 4, pages: 261-267
Publisher: SPRINGER-VERLAG

SLU Authors

• Andersson, Mariette

  • Plant Science Sweden AB

• Hofvander, Per

  • Plant Science Sweden AB
  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Plant Biotechnology

Publication identifier

• DOI: https://doi.org/10.1007/s00299-003-0684-8

Permanent link to this page (URI)

https://res.slu.se/id/publ/51964