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Research article - Peer-reviewed, 2014

Genetic diversity in ex-situ conserved sorghum accessions of Botswana as estimated by microsatellite markers

Motlhaodi, Tiny; Geleta, Mulatu Dida; Bryngelsson, Tomas; Fatih, Moneim; Chite, S.; Ortiz Rios, Rodomiro Octavio

Abstract

Simple sequence repeat (SSR) markers were used to characterize genetic diversity in 30 sorghum [Sorghum bicolor (L.) Moench] accessions conserved in the national gene bank of Botswana. This collection represents the three sorghum-growing agro-ecological zones in the country. Ten microsatellite primer pairs were used, and these generated a total of 53 alleles with three to seventeen alleles per locus across the 30 accessions. A high level of genetic variation was observed among the accessions (70% of the variation), and 30% of the total genetic variation was observed within accessions. The analysis of molecular variance on sorghum accessions grouped based on agro-ecological region, ethnicity, and sorghum race revealed a non-significant variation, indicating that the alleles are distributed all over the country. Cluster analysis also did not group the accessions according to agro-ecological region, race or ethnicity. The results of this study have indicated that substantial genetic diversity exists among sorghum accessions conserved in Botswana, but that agro-ecological region, ethnicity and race did not influence the distribution of this diversity

Published in

Australian Journal Of Crop Science
2014, volume: 8, number: 1, pages: 35-43

Authors' information

Motlhaodi, Tiny
Swedish University of Agricultural Sciences, Department of Plant Breeding
Geleta, Mulatu Dida (Dida, Mulatu Geleta)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Bryngelsson, Tomas
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Breeding
Chite, S.
Swedish University of Agricultural Sciences, Department of Plant Breeding

UKÄ Subject classification

Genetics and Breeding

URI (permanent link to this page)

https://res.slu.se/id/publ/52191