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Research article - Peer-reviewed, 2014

Neuropeptides in the Antennal Lobe of the Yellow Fever Mosquito, Aedes aegypti.

Purayil, Siju; Reifenrath, Anna; Scheiblich, Hannah; Neupert, Susanne; Predel, Reinhard; Hansson, Bill; Schachtner, Joachim; Ignell, Rickard

Abstract

For many insects, including mosquitoes, olfaction is the dominant modality regulating their behavioral repertoire. Many neurochemicals modulate olfactory information in the central nervous system, including the primary olfactory center of insects, the antennal lobe. The most diverse and versatile neurochemicals in the insect nervous system are found in the neuropeptides. In the present study, we analyzed neuropeptides in the antennal lobe of the yellow fever mosquito, Aedes aegypti, a major vector of arboviral diseases. Direct tissue profiling of the antennal lobe by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry indicated the presence of 28 mature products from 10 different neuropeptide genes. In addition, immunocytochemical techniques were used to describe the cellular location of the products of up to seven of these genes within the antennal lobe. Allatostatin A, allatotropin, SIFamide, FMRFamide-related peptides, short neuropeptide F, myoinhibitory peptide, and tachykinin-related peptides were found to be expressed in local interneurons and extrinsic neurons of the antennal lobe. Building on these results, we discuss the possible role of neuropeptide signaling in the antennal lobe of Ae. aegypti. J. Comp. Neurol. 522:592-608, 2014. (c) 2013 Wiley Periodicals, Inc.

Keywords

olfaction; neuromodulation; mass spectrometry; immunocytochemistry; insect brain

Published in

Journal of Comparative Neurology
2014, volume: 522, number: 3, pages: 592-608
Publisher: WILEY-BLACKWELL

Authors' information

Purayil, Siju
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Reifenrath, Anna
Philipps University Marburg
Scheiblich, Hannah
Philipps University Marburg
Neupert, Susanne
University of Cologne
Predel, Reinhard
University of Cologne
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Schachtner, Joachim
Philipps University Marburg
Swedish University of Agricultural Sciences, Department of Plant Protection Biology

UKÄ Subject classification

Other Biological Topics
Cell Biology
Structural Biology
Zoology

Publication Identifiers

DOI: https://doi.org/10.1002/cne.23434

URI (permanent link to this page)

https://res.slu.se/id/publ/52218