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Abstract

We have characterized three CoA-independent types of enzyme, phospholipases, phospholipid: diacylglycerol acyltransferases (PDATs) and cholinephosphotransferases, responsible for the removal of unusual fatty acids from phosphatidylcholine (PC) in microsomal preparations from developing oil seeds. The metabolism of sn-2-[C-14]acyl-PC was monitored in microsomal preparations from various oilseeds having either medium-chain, acetylenic, epoxy or hydroxy fatty acids as their major fatty acids in the oil. The results indicate that PDAT plays a major role in removing ricinoleic acid and vernolic acid from phospholipids in Ricinus communis and Crepis palaestina seeds, respectively. However, vernolic, crepenynic and capric acids are primarily removed from phospholipids by phospholipases in Euphorbia lagascae, Crepis rubra and elm seeds, respectively. Further, we show that significant PDAT activity is also present in vegetative tissues of Arabidopsis thaliana.

Keywords

cholinephosphotransferase; lipid; phospholipases; phospholipid : diacylglycerol acyl transferase; triacylglycerol

Published in

Biochemical Society Transactions
2000, volume: 28, pages: 703-705
Publisher: PORTLAND PRESS

SLU Authors

  • Banas, Antoni

    • Scandinavian Biotechnology Research (ScanBiRes) AB

  • Dahlqvist, Anders

    • Scandinavian Biotechnology Research (ScanBiRes) AB

  • Stymne, Sten

    • Department of Plant Breeding Research, Swedish University of Agricultural Sciences

UKÄ Subject classification

Cell Biology

Publication identifier

  • DOI: https://doi.org/10.1042/BST0280703

Permanent link to this page (URI)

https://res.slu.se/id/publ/52288