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Research article2000Peer reviewed

The involvement of phospholipid : diacylglycerol acyltransferases in triacylglycerol production

Banas, Antoni; Dahlqvist, Anders; Ståhl, U.; Lenman, Marit; Stymne, Sten


We have characterized three CoA-independent types of enzyme, phospholipases, phospholipid: diacylglycerol acyltransferases (PDATs) and cholinephosphotransferases, responsible for the removal of unusual fatty acids from phosphatidylcholine (PC) in microsomal preparations from developing oil seeds. The metabolism of sn-2-[C-14]acyl-PC was monitored in microsomal preparations from various oilseeds having either medium-chain, acetylenic, epoxy or hydroxy fatty acids as their major fatty acids in the oil. The results indicate that PDAT plays a major role in removing ricinoleic acid and vernolic acid from phospholipids in Ricinus communis and Crepis palaestina seeds, respectively. However, vernolic, crepenynic and capric acids are primarily removed from phospholipids by phospholipases in Euphorbia lagascae, Crepis rubra and elm seeds, respectively. Further, we show that significant PDAT activity is also present in vegetative tissues of Arabidopsis thaliana.


cholinephosphotransferase; lipid; phospholipases; phospholipid : diacylglycerol acyl transferase; triacylglycerol

Published in

Biochemical Society Transactions
2000, Volume: 28, pages: 703-705

            SLU Authors

          • Stymne, Sten

            • Department of Plant Breeding Research, Swedish University of Agricultural Sciences

          UKÄ Subject classification

          Cell Biology

          Publication identifier


          Permanent link to this page (URI)