Research article - Peer-reviewed, 2000
An acyl-CoA : cholesterol acyltransferase (ACAT)-related gene is involved in the accumulation of triacylglycerols in Saccharomyces cerevisiae
Sandager, Line; Dahlqvist, Anders; Banas, Antoni; Ståhl, U.; Lenman, Marit; Gustavsson, M.; Stymne, StenAbstract
The major route for the synthesis of triacylglycerol (TAG) in yeast as well as in all TAG-accumulating organisms has been suggested to occur via the acylation of diacylglycerol (DAG) by acyl-CoA:diacylglycerol acyltransferase (DAGAT). Genes encoding DAGAT have been identified in both plant and animal tissues. These genes show strong sequence similarities to genes encoding acyl-CoA: cholesterol acyltransferase (ACAT), So far no Saccharomyces cerevisiae DAGAT gene has been published; however, two ACAT-like genes, ARE1 and ARE2, are present in the yeast genome. Both these genes have been suggested to be involved in the synthesis of sterol esters. We have now shown that the ARE1 gene in yeast also is involved in the synthesis of TAG, whereas the ARE2 gene is more specifically involved in the synthesis of sterol esters.Keywords
ACAT; DAGAT; lipid; TAG; yeastPublished in
Biochemical Society Transactions2000, volume: 28, pages: 700-702
Publisher: PORTLAND PRESS
Authors' information
Sandager, Line
Swedish University of Agricultural Sciences, Department of Plant Breeding Research
Dahlqvist, Anders
Scandinavian Biotechnology Research (ScanBiRes) AB
Banas, Antoni
Scandinavian Biotechnology Research (ScanBiRes) AB
Ståhl, U.
Scandinavian Biotechnology Research (ScanBiRes) AB
Scandinavian Biotechnology Research (ScanBiRes) AB
Gustavsson, M.
SLU - Swedish University of Agricultural Sciences
Swedish University of Agricultural Sciences, Department of Plant Breeding Research
UKÄ Subject classification
Plant Biotechnology
Publication Identifiers
DOI: https://doi.org/10.1042/BST0280700
URI (permanent link to this page)
https://res.slu.se/id/publ/52289