Lenman, Marit
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
Research article1998Peer reviewed
Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation
Lee, Michael; Lenman, Marit; Banas, Antoni; Bafor, Maureen; Surinder, Singh; Schweizer, Michael; Nilsson, Ralf; Liljenberg, Conny; Dahlqvist, Anders; Gummeson, Per-Olov; Sjödahl, Staffan; Green, Allan; Stymne, Sten
Abstract
Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta 12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta 12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.
Published in
Science
1998, Volume: 280, number: 5365, pages: 915-918
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Banas, Antoni
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
Dahlqvist, Anders
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
Stymne, Sten
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
- Department of Plant Breeding Research, Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Plant Biotechnology
Cell Biology
Publication identifier
DOI: https://doi.org/10.1126/science.280.5365.915
Permanent link to this page (URI)
https://res.slu.se/id/publ/52295