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Research article - Peer-reviewed, 1992

The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family

Xue, Jiaping; Lenman, Marit; Falk, Anders; Rask, Lars

Abstract

A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.

Keywords

ARABIDOPSIS-THALIANA; BRASSICACEAE; BRASSICA-NAPUS; GLUCOSINOLATE; MYROSINASE; SINAPIS-ALBA

Published in

Plant Molecular Biology
1992, volume: 18, number: 2, pages: 387-398
Publisher: KLUWER ACADEMIC PUBL

Authors' information

Xue, Jiaping
SLU - Swedish University of Agricultural Sciences
Swedish University of Agricultural Sciences, Department of Cell Research
Falk, Anders
Swedish University of Agricultural Sciences, Department of Cell Research
Rask, Lars
Swedish University of Agricultural Sciences, Department of Cell Research

UKÄ Subject classification

Plant Biotechnology

Publication Identifiers

DOI: https://doi.org/10.1007/BF00034965

URI (permanent link to this page)

https://res.slu.se/id/publ/52303