Skip to main content
SLU publication database (SLUpub)
Research article - Peer-reviewed, 1992

The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family

Xue, Jiaping; Lenman, Marit; Falk, Anders; Rask, Lars

Abstract

A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.

Keywords

ARABIDOPSIS-THALIANA; BRASSICACEAE; BRASSICA-NAPUS; GLUCOSINOLATE; MYROSINASE; SINAPIS-ALBA

Published in

Plant Molecular Biology
1992, Volume: 18, number: 2, pages: 387-398
Publisher: KLUWER ACADEMIC PUBL

      SLU Authors

    • Lenman, Marit

      • Department of Cell Research, Swedish University of Agricultural Sciences
      • Falk, Anders

        • Department of Cell Research, Swedish University of Agricultural Sciences
        • Rask, Lars

          • Department of Cell Research, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Plant Biotechnology

        Publication identifier

        DOI: https://doi.org/10.1007/BF00034965

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/52303