Myrosinase is localized to the interior of myrosin grains and is not associated to the surrounding tonoplast membrane

Höglund, Anna-Stina; Lenman, Marit; Rask, Lars

doi:10.1016/0168-9452(92)90112-Y

Research article - Peer-reviewed, 1992

Myrosinase is localized to the interior of myrosin grains and is not associated to the surrounding tonoplast membrane

Höglund, Anna-Stina; Lenman, Marit; Rask, Lars

Abstract

The distribution of myrosinase was investigated in embryos of different age of Sinapis alba by immuno electron microscopy. Using a well characterized monoclonal antibody the myrosinase was found to be exclusively localized to the interior of the myrosin grains, a finding in contrast to earlier studies by light microscopy which indicated either a cytoplasmic distribution or an association to the tonoplast membrane surrounding the myrosin grains.

Keywords

SINAPIS-ALBA; MYROSINASE; MYROSIN GRAINS; IMMUNOGOLD LOCALIZATION BY MONOCLONAL ANTIBODIES

Published in

Plant Science
1992, volume: 85, number: 2, pages: 165-170
Publisher: ELSEVIER SCI IRELAND LTD

Authors' information

Höglund, Anna-Stina

Swedish University of Agricultural Sciences, Department of Cell Research

Lenman, Marit

Swedish University of Agricultural Sciences, Department of Cell Research

Rask, Lars

Swedish University of Agricultural Sciences, Department of Cell Research

UKÄ Subject classification

Cell Biology

Publication Identifiers

DOI: https://doi.org/10.1016/0168-9452(92)90112-Y

URI (permanent link to this page)

https://res.slu.se/id/publ/52304