Abstract

Immunocytochemical studies on Brassica napus (rapeseed) tissues using a monoclonal antibody against myrosinase thioglucoside glucohydrolase) showed that the enzyme was only present in a small number of cells. In the developing embryo, scattered myrosinase-containing cells were present in both cotyledons and axis. The enzyme accumulated in these cells during the later stages of seed development, approximately from day 20 until day 40 after pollination. Parallel staining with the immunocytochemical technique and a histochemical method identified these cells as myrosin cells. Myrosinase appeared to be located outside the myrosin grains, although the occasional association with the membrane of the grains also was noted. In leaves, petals, and siliques, scattered parenchyma cells were stained in the mesophyll as well as in the vascular tissue. In young leaves, guard cells also contained myrosinase. The enzyme was also present in xylem cells of the stem.

Published in

Plant Physiology
1991, Volume: 95, number: 1, pages: 213-221
Publisher: AMER SOC PLANT PHYSIOLOGISTS

SLU Authors

• Höglund, Anna-Stina

  • Department of Cell Research, Swedish University of Agricultural Sciences
    

• Lenman, Marit

  • Department of Cell Research, Swedish University of Agricultural Sciences
    

• Falk, Anders

  • Department of Cell Research, Swedish University of Agricultural Sciences
    

• Rask, Lars

  • Department of Cell Research, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology


Publication identifier

DOI: https://doi.org/10.1104/pp.95.1.213

Permanent link to this page (URI)

https://res.slu.se/id/publ/52305