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Research article - Peer-reviewed, 2013

ROOT ULTRAVIOLET B-SENSITIVE1/WEAK AUXIN RESPONSE3 Is Essential for Polar Auxin Transport in Arabidopsis

Yu, Hong; Karampelias, Michael; Robert, Stephanie; Peer, Wendy Ann; Swarup, Ranjan; Ye, Songqing; Ge, Lei; Cohen, Jerry; Murphy, Angus; Friml, Jiri; Estelle, Mark

Abstract

The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak auxin response3 (wxr3), exhibits much lower DR5rev: GFP expression when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases polar auxin transport and results in a disruption of the asymmetric auxin distribution. The levels of the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of polar auxin transport by maintaining the proper level of auxin transporters on the plasma membrane.

Published in

Plant Physiology
2013, Volume: 162, number: 2, pages: 965-976
Publisher: AMER SOC PLANT BIOLOGISTS