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Research article - Peer-reviewed, 2013

Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana

Tanaka, Hirokazu; Kitakura, Saeko; Rakusova, Hana; Uemura, Tomohiro; Feraru, Mugurel I.; De Rycke, Riet; Robert, Stephanie; Kakimoto, Tatsuo; Friml, Jiri

Abstract

PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but mechanisms regulating PIN localization are not fully understood. Here we show that early endosomal components ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively required for polar PIN localization, for their dynamic repolarization, and consequently for auxin activity gradient formation and auxin-related developmental processes including embryonic patterning, organogenesis, and vasculature venation patterning. These results show that early endosomal trafficking is crucial for cell polarity and auxin-dependent regulation of plant architecture.

Published in

PLoS Genetics
2013, Volume: 9, number: 5, article number: e1003540
Publisher: PUBLIC LIBRARY SCIENCE

    UKÄ Subject classification

    Developmental Biology
    Cell Biology

    Publication identifier

    DOI: https://doi.org/10.1371/journal.pgen.1003540

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/52723