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Research article - Peer-reviewed, 2012

ABP1 and ROP6 GTPase Signaling Regulate Clathrin-Mediated Endocytosis in Arabidopsis Roots

Chen, Xu; Naramoto, Satoshi; Robert, Stephanie; Tejos, Ricardo; Loefke, Christian; Lin, Deshu; Yang, Zhenbiao; Friml, Jiri

Abstract

The dynamic spatial and temporal distribution of the crucial plant signaling molecule auxin is achieved by feedback coordination of auxin signaling and intercellular auxin transport pathways [1, 2]. Developmental roles of auxin have been attributed predominantly to its effect on transcription; however, an alternative pathway involving AUXIN BINDING PROTEIN1 (ABP1) has been proposed to regulate clathrin-mediated endocytosis in roots and Rho-like GTPase (ROP)-dependent pavement cell interdigitation in leaves [3, 4]. In this study, we show that ROP6 and its downstream effector RIC1 regulate clathrin association with the plasma membrane for clathrin-mediated endocytosis, as well as for its feedback regulation by auxin. Genetic analysis revealed that ROP6/RIC1 acts downstream of ABP1 to regulate endocytosis. This signaling circuit is also involved in the feedback regulation of PIN-FORMED 1 (PIN1) and PIN2 auxin transporters activity (via its constitutive endocytosis) and corresponding auxin transport-mediated processes, including root gravitropism and leave vascular tissue patterning. Our findings suggest that the signaling module auxin ABP1 ROP6/RIC1 clathrin PIN1/PIN2 is a shared component of the feedback regulation of auxin transport during both root and aerial development.

Published in

Current Biology
2012, Volume: 22, number: 14, pages: 1326-1332
Publisher: CELL PRESS

    UKÄ Subject classification

    Developmental Biology
    Cell Biology

    Publication identifier

    DOI: https://doi.org/10.1016/j.cub.2012.05.020

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/52726