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Research article - Peer-reviewed, 2013

Catalase and NO CATALASE ACTIVITY1 Promote Autophagy-Dependent Cell Death in Arabidopsis

Hackenberg, Thomas; Juul, Trine; Auzina, Aija; Gwizdz, Sonia; Malolepszy, Anna; Van der Kelen, Katrien; Dam, Svend; Bressendorff, Simon; Lorentzen, Andrea; Roepstorff, Peter; Nielsen, Kare Lehmann; Jorgensen, Jan-Elo; Hofius, Daniel; Van Breusegem, Frank; Petersen, Morten; Andersen, Stig Uggerhoj


Programmed cell death often depends on generation of reactive oxygen species, which can be detoxified by antioxidative enzymes, including catalases. We previously isolated catalase-deficient mutants (cat2) in a screen for resistance to hydroxyurea-induced cell death. Here, we identify an Arabidopsis thaliana hydroxyurea-resistant autophagy mutant, atg2, which also shows reduced sensitivity to cell death triggered by the bacterial effector avrRpm1. To test if catalase deficiency likewise affected both hydroxyurea and avrRpm1 sensitivity, we selected mutants with extremely low catalase activities and showed that they carried mutations in a gene that we named NO CATALASE ACTIVITY1 (NCA1). nca1 mutants showed severely reduced activities of all three catalase isoforms in Arabidopsis, and loss of NCA1 function led to strong suppression of RPM1-triggered cell death. Basal and starvation-induced autophagy appeared normal in the nca1 and cat2 mutants. By contrast, autophagic degradation induced by avrRpm1 challenge was compromised, indicating that catalase acted upstream of immunity-triggered autophagy. The direct interaction of catalase with reactive oxygen species could allow catalase to act as a molecular link between reactive oxygen species and the promotion of autophagy-dependent cell death.

Published in

Plant Cell
2013, volume: 25, number: 11, pages: 4616-4626

Authors' information

Hackenberg, Thomas
Juul, Trine
Auzina, Aija
Gwizdz, Sonia
Malolepszy, Anna
Van der Kelen, Katrien
Ghent University
Dam, Svend
Bressendorff, Simon
Lorentzen, Andrea
Roepstorff, Peter
Nielsen, Kare Lehmann
Jorgensen, Jan-Elo
Swedish University of Agricultural Sciences, Department of Plant Biology
University of Copenhagen
Van Breusegem, Frank
Ghent University
Petersen, Morten
Andersen, Stig Uggerhoj

UKÄ Subject classification

Developmental Biology
Evolutionary Biology

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