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Research article - Peer-reviewed, 2013

ECHIDNA-mediated post-Golgi trafficking of auxin carriers for differential cell elongation

Boutté, Yohann; Jonsson, Kristoffer; McFarlane, Heather E.; Johnson, Errin; Gendre, Delphine; Swarup, Ranjan; Friml, Jiří; Samuels, Lacey; Robert, Stephanie; Bhalerao, Rishikesh P.

Abstract

The plant hormone indole-acetic acid (auxin) is essential for many aspects of plant development. Auxin-mediated growth regulation typically involves the establishment of an auxin concentration gradient mediated by polarly localized auxin transporters. The localization of auxin carriers and their amount at the plasma membrane are controlled by membrane trafficking processes such as secretion, endocytosis, and recycling. In contrast to endocytosis or recycling, how the secretory pathway mediates the localization of auxin carriers is not well understood. In this study we have used the differential cell elongation process during apical hook development to elucidate the mechanisms underlying the post-Golgi trafficking of auxin carriers in Arabidopsis. We show that differential cell elongation during apical hook development is defective in Arabidopsis mutant echidna (ech). ECH protein is required for the trans-Golgi network (TGN)-mediated trafficking of the auxin influx carrier AUX1 to the plasma membrane. In contrast, ech mutation only marginally perturbs the trafficking of the highly related auxin influx carrier LIKE-AUX1-3 or the auxin efflux carrier PIN-FORMED-3, both also involved in hook development. Electron tomography reveals that the trafficking defects in ech mutant are associated with the perturbation of secretory vesicle genesis from the TGN. Our results identify differential mechanisms for the post-Golgi trafficking of de novo-synthesized auxin carriers to plasma membrane from the TGN and reveal how trafficking of auxin influx carriers mediates the control of differential cell elongation in apical hook development.

Keywords

sorting; IAA; morphogenesis

Published in

Proceedings of the National Academy of Sciences
2013, volume: 110, number: 40, pages: 16259-16264
Publisher: NATL ACAD SCIENCES

Authors' information

Boutté, Yohann
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
McFarlane, Heather E.
University of British Columbia
Johnson, Errin
No organisation
Gendre, Delphine
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swarup, Ranjan
University of Nottingham
Friml, Jiří
Institute of Science and Technology Austria
Samuels, Lacey
University of British Columbia
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Botany

Publication Identifiers

DOI: https://doi.org/10.1073/pnas.1309057110

URI (permanent link to this page)

https://res.slu.se/id/publ/52976