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Research article - Peer-reviewed, 2014

Osteochondral lesions in distal tarsal joints of Icelandic horses reveal strong associations between hyaline and calcified cartilage abnormalities.

Ley, Charles; Ekman, Stina; Hansson, Kerstin; Björnsdottir, Sigridur; Boyde, Alan; Björnsdottir, S; Boyde, Alan

Abstract

Osteochondral lesions in the joints of the distal tarsal region of young Icelandic horses provide a natural model for the early stages of osteoarthritis (OA) in low-motion joints. We describe and characterise mineralised and non-mineralised osteochondral lesions in left distal tarsal region joint specimens from twenty-two 30 ±1 month-old Icelandic horses. Combinations of confocal scanning light microscopy, backscattered electron scanning electron microscopy (including, importantly, iodine staining) and three-dimensional microcomputed tomography were used on specimens obtained with guidance from clinical imaging. Lesion-types were described and classified into groups according to morphological features. Their locations in the hyaline articular cartilage (HAC), articular calcified cartilage (ACC), subchondral bone (SCB) and the joint margin tissues were identified and their frequency in the joints recorded. Associations and correlations between lesion-types were investigated for centrodistal joints only. In centrodistal joints the lesion-types HAC chondrocyte loss, HAC fibrillation, HAC central chondrocyte clusters, ACC arrest and ACC advance had significant associations and strong correlations. These lesion-types had moderate to high frequency in centrodistal joints but low frequencies in tarsometatarsal and talocalcaneal-centroquartal joints. Joint margin lesion-types had no significant associations with other lesion-types in the centrodistal joints but high frequency in both the centrodistal and tarsometatarsal joints. The frequency of SCB lesion-types in all joints was low. Hypermineralised infill phase lesion-types were detected. Our results emphasise close associations between HAC and ACC lesions in equine centrodistal joints and the importance of ACC lesions in the development of OA in low-motion compression-loaded equine joints.

Keywords

articular cartilage; subchondral bone; osteoarthritis; equine tarsus; back scattered electron microscopy; iodine-staining; confocal scanning light microscopy; microcomputed tomography

Published in

eCells and Materials Journal
2014, volume: 27, pages: 213-236

Authors' information

Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Björnsdottir, Sigridur (Björnsdottir, Sigridur)
Boyde, Alan
Björnsdottir, S (Björnsdottir, S)
Icelandic Food and Veterinary Authority
Boyde, Alan
Queen Mary University of London

UKÄ Subject classification

Orthopedics
Pathobiology
Cell and Molecular Biology
Clinical Science
Other Veterinary Science

Publication Identifiers

DOI: https://doi.org/10.22203/eCM.v027a16

URI (permanent link to this page)

https://res.slu.se/id/publ/53082