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Research article - Peer-reviewed, 2002

Crystallization and X-ray analysis of native and selenome­thionyl β-mannanase Man5A from blue mussel, Mytilus edulis, expressed in Pichia pastoris

Xu, Bingze; Munoz, Ines G.; Janson, Jan-Christer; Ståhlberg, Jerry


The glycohydrolase family 5 beta-mannanase Man5A from Mytilus edulis has been expressed in Pichia pastoris and purified in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop technique at 293 K using polyethylene glycol 5000 monomethylether as precipitant and dioxane as additive. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.8, b = 64.8, c = 90.7 Angstrom. Diffraction to 1.4 Angstrom resolution has been obtained at 100 K. Expression was also performed in the presence of selenomethionine. The incorporation of SeMet was estimated at 40% by amino-acid analysis and its presence in crystals was confirmed from the X-ray absorption scanning spectrum.

Published in

Acta Crystallographica Section D: Biological Crystallography
2002, volume: 58, pages: 542-545

Authors' information

Xu, Bingze
Uppsala University
Munoz, Ines G.
Swedish University of Agricultural Sciences, Department of Molecular Biology
Janson, Jan-Christer
Uppsala University
Swedish University of Agricultural Sciences, Department of Molecular Biology

UKÄ Subject classification

Structural Biology

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