Research article - Peer-reviewed, 2002
Crystallization and X-ray analysis of native and selenomethionyl β-mannanase Man5A from blue mussel, Mytilus edulis, expressed in Pichia pastoris
Xu, Bingze; Munoz, Ines G.; Janson, Jan-Christer; Ståhlberg, JerryAbstract
The glycohydrolase family 5 beta-mannanase Man5A from Mytilus edulis has been expressed in Pichia pastoris and purified in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop technique at 293 K using polyethylene glycol 5000 monomethylether as precipitant and dioxane as additive. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.8, b = 64.8, c = 90.7 Angstrom. Diffraction to 1.4 Angstrom resolution has been obtained at 100 K. Expression was also performed in the presence of selenomethionine. The incorporation of SeMet was estimated at 40% by amino-acid analysis and its presence in crystals was confirmed from the X-ray absorption scanning spectrum.Published in
Acta Crystallographica Section D: Biological Crystallography2002, volume: 58, pages: 542-545
Publisher: BLACKWELL MUNKSGAARD
Authors' information
Xu, Bingze
Uppsala University
Munoz, Ines G.
Swedish University of Agricultural Sciences, Department of Molecular Biology
Janson, Jan-Christer
Uppsala University
Swedish University of Agricultural Sciences, Department of Molecular Biology
UKÄ Subject classification
Structural Biology
Publication Identifiers
DOI: https://doi.org/10.1107/S0907444902000355
URI (permanent link to this page)
https://res.slu.se/id/publ/53155