Abstract

Plant cell-wall polysaccharides represent a vast source of food in nature. To depolymerize polysaccharides to soluble sugars, many organisms use multifunctional enzyme mixtures consisting of glycoside hydrolases, lytic polysaccharide mono-oxygenases, polysaccharide lyases, and carbohydrate esterases, as well as accessory, redox-active enzymes for lignin depolymerization. Many of these enzymes that degrade lignocellulose are multimodular with carbohydrate-binding modules (CBMs) and catalytic domains connected by flexible, glycosylated linkers. These linkers have long been thought to simply serve as a tether between structured domains or to act in an inchworm-like fashion during catalytic action. To examine linker function, we performed molecular dynamics (MD) simulations of the Trichoderma reesei Family 6 and Family 7 cellobiohydrolases (TrCel6A and TrCel7A, respectively) bound to cellulose. During these simulations, the glycosylated linkers bind directly to cellulose, suggesting a previously unknown role in enzyme action. The prediction from the MD simulations was examined experimentally by measuring the binding affinity of the Cel7A CBM and the natively glycosylated Cel7A CBM-linker. On crystalline cellulose, the glycosylated linker enhances the binding affinity over the CBM alone by an order of magnitude. The MD simulations before and after binding of the linker also suggest that the bound linker may affect enzyme action due to significant damping in the enzyme fluctuations. Together, these results suggest that glycosylated linkers in carbohydrate-active enzymes, which are intrinsically disordered proteins in solution, aid in dynamic binding during the enzymatic deconstruction of plant cell walls.

Keywords

biofuels; cellulase; post-translational modification; carbohydrate recognition

Published in

Proceedings of the National Academy of Sciences
2013, Volume: 110, number: 36, pages: 14646-14651
Publisher: NATL ACAD SCIENCES

SLU Authors

• Sandgren, Mats

  • Department of Molecular Biology, Swedish University of Agricultural Sciences
    

• Ståhlberg, Jerry

  • Department of Molecular Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Structural Biology
Renewable Bioenergy Research
Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1073/pnas.1309106110

Permanent link to this page (URI)

https://res.slu.se/id/publ/53159