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Research article - Peer-reviewed, 2002

Preparation and crystallization of selenomethionyl dextranase from Penicillium minioluteum expressed in Pichia pastoris

Larsson, Anna M.; Ståhlberg, Jerry; Jones, T. Alwyn

Abstract

Dextranase from the fungus Penicillium minioluteum hydrolyses alpha-1,6-glycosidic bonds in dextran polymers. The enzyme has been expressed in Pichia pastoris in the presence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by amino-acid analysis to be 50%. The protein has been crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 103.6, b = 115.3, c = 49.8 Angstrom and one molecule per asymmetric unit. The crystals diffract to 2.0 Angstrom and the presence of SeMet in the crystals has been confirmed by an X-ray absorption spectrum.

Published in

Acta Crystallographica Section D: Biological Crystallography
2002, Volume: 58, pages: 346-348
Publisher: BLACKWELL MUNKSGAARD

      SLU Authors

    • Ståhlberg, Jerry

      • Department of Molecular Biology, Swedish University of Agricultural Sciences

    UKÄ Subject classification

    Biochemistry and Molecular Biology
    Structural Biology

    Publication identifier

    DOI: https://doi.org/10.1107/S0907444901020406

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/53162