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Research article - Peer-reviewed, 2002

Preparation and crystallization of selenomethionyl dextranase from Penicillium minioluteum expressed in Pichia pastoris

Larsson, Anna M.; Ståhlberg, Jerry; Jones, T. Alwyn


Dextranase from the fungus Penicillium minioluteum hydrolyses alpha-1,6-glycosidic bonds in dextran polymers. The enzyme has been expressed in Pichia pastoris in the presence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by amino-acid analysis to be 50%. The protein has been crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 103.6, b = 115.3, c = 49.8 Angstrom and one molecule per asymmetric unit. The crystals diffract to 2.0 Angstrom and the presence of SeMet in the crystals has been confirmed by an X-ray absorption spectrum.

Published in

Acta Crystallographica Section D: Biological Crystallography
2002, volume: 58, pages: 346-348

Authors' information

Larsson, Anna M.
Uppsala University
Swedish University of Agricultural Sciences, Department of Molecular Biology
Jones, T. Alwyn
Uppsala University

UKÄ Subject classification

Biochemistry and Molecular Biology
Structural Biology

Publication Identifiers


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