Research article - Peer-reviewed, 2002
Preparation and crystallization of selenomethionyl dextranase from Penicillium minioluteum expressed in Pichia pastorisLarsson, Anna M.; Ståhlberg, Jerry; Jones, T. Alwyn
AbstractDextranase from the fungus Penicillium minioluteum hydrolyses alpha-1,6-glycosidic bonds in dextran polymers. The enzyme has been expressed in Pichia pastoris in the presence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by amino-acid analysis to be 50%. The protein has been crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 103.6, b = 115.3, c = 49.8 Angstrom and one molecule per asymmetric unit. The crystals diffract to 2.0 Angstrom and the presence of SeMet in the crystals has been confirmed by an X-ray absorption spectrum.
Published inActa Crystallographica Section D: Biological Crystallography
2002, volume: 58, pages: 346-348
Publisher: BLACKWELL MUNKSGAARD
Larsson, Anna M.
Swedish University of Agricultural Sciences, Department of Molecular Biology
Jones, T. Alwyn
UKÄ Subject classification
Biochemistry and Molecular Biology
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