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Research article - Peer-reviewed, 2013

The structure and immunoreactivity of exopolysaccharide isolated from Lactobacillus johnsonii strain 151

Gorska-Fraczek, Sabina; Sandström, Corine; Kenne, Lennart; Pasciak, Mariola; Brzozowska, Ewa; Strus, Magdalena; Heczko, Piotr; Gamian, Andrzej


The immunoreactivity of two structurally different exopolysaccharides isolated from L. johnsonii, 151 and 142 (Carbohydr. Res. 2010, 345, 108-114), was compared. Both EPSs differed in their reactivity with antisera. EPS from L. johnsonii 151 reacted with anti-Lactobacillus polyclonal sera against cells of five different strains, while EPS from L. johnsonii 142 was found to react only with its own antiserum. The broader specificity and higher reactivity of EPS from 151 strain than EPS from 142 strain were also observed with human sera. The physiological antibodies recognizing polysaccharide antigens were present in both adults and umbilical cord blood sera. A highly specific EPS 142 bearing strain was isolated from experimentally induced inflammatory bowel disease (IBD) mice, while a strain with EPS 151 isolated from the intestinal tract of healthy mice is characterized by a broad immune reactivity common structure. (C) 2013 Elsevier Ltd. All rights reserved.


Lactobacillus; Exopolysaccharide structure; Immunoreactivity; NMR

Published in

Carbohydrate Research
2013, Volume: 378, pages: 148-153