Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructure

Hilden L, Daniel G, Johansson G

doi:10.1023/A:1022846304826

Research article - Peer-reviewed, 2003

Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructure

Hilden L, Daniel G, Johansson G

Abstract

The alpha-amino group of the carbohydrate-binding module (CBM) from Phanerochaete chrysosporium cellulase Cel7D was covalently labelled with fluorescein isothiocyanate. The fluorescein-labelled CBM was characterised regarding substrate binding, showing specificity only to cellulose and not to mannan and xylan. Conjugation of fluorescein isothiocyanate to CBM did not affect its binding to cellulose. The labelled CBM was successfully used as a probe for detecting cellulose in lignocellulose material such as never dried spruce and birch wood as well as pulp fibres

Published in

Biotechnology Letters
2003, volume: 25, number: 7, pages: 553-558
Publisher: KLUWER ACADEMIC PUBL

Authors' information

Hilden, Lars

Swedish University of Agricultural Sciences, Forest economy

Daniel, Geoffrey

Swedish University of Agricultural Sciences, Department of Forest Products

Johansson, G

UKÄ Subject classification

Forest Science

Publication Identifiers

DOI: https://doi.org/10.1023/A:1022846304826

URI (permanent link to this page)

https://res.slu.se/id/publ/542