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Research article2003Peer reviewed

Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructure

Hilden L, Daniel G, Johansson G

Abstract

The alpha-amino group of the carbohydrate-binding module (CBM) from Phanerochaete chrysosporium cellulase Cel7D was covalently labelled with fluorescein isothiocyanate. The fluorescein-labelled CBM was characterised regarding substrate binding, showing specificity only to cellulose and not to mannan and xylan. Conjugation of fluorescein isothiocyanate to CBM did not affect its binding to cellulose. The labelled CBM was successfully used as a probe for detecting cellulose in lignocellulose material such as never dried spruce and birch wood as well as pulp fibres

Published in

Biotechnology Letters
2003, Volume: 25, number: 7, pages: 553-558
Publisher: KLUWER ACADEMIC PUBL

      SLU Authors

    • Hilden, Lars

      • Department of Forest Products, Swedish University of Agricultural Sciences

      • Daniel, Geoffrey

        • Department of Forest Products, Swedish University of Agricultural Sciences

      UKÄ Subject classification

      Forest Science

      Publication identifier

      DOI: https://doi.org/10.1023/A:1022846304826

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/542