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Research article2013Peer reviewedOpen access

A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa

seong-bin, Kim ; Timmusk, Salme

Abstract

Conclusions: We have created a simple system for targeted gene deletion in P. polymyxa E681. We propose that P. polymyxa isogenic mutants could be developed using this system of marker exchange mutagenesis. alpha- and beta-amylase genes provide a useful tool for direct recombinant screening in P. polymyxa.

Published in

PLoS ONE
2013, Volume: 8, number: 6
Publisher: PUBLIC LIBRARY SCIENCE