Research article - Peer-reviewed, 2013
Treatment Efficacy and Immune Stimulation by AdCD40L Gene Therapy of Spontaneous Canine Malignant Melanoma
Westberg, Sara; Sadeghi, Arian; Svensson, Emma; Segall, Thomas; Dimopoulou, Maria; Korsgren, Olle; Hemminki, Akseli; Loskog, Angelica S. I.; Tötterman, Thomas H.; Von Euler, HenrikAbstract
Malignant melanoma is a serious disease in both humans and dogs, and the high metastatic potential results in poor prognosis for many patients. Its similarities with human melanoma make spontaneous canine melanoma an excellent model for comparative studies of novel therapies and tumor biology. We report a pilot study of local adenovector CD40L (AdCD40L) immunogene treatment in 19 cases of canine melanoma (14 oral, 4 cutaneous, and 1 conjunctival). Three patients were World Health Organization stage I, 2 were stage II, 10 stage III, and 4 stage IV. One to 6 intratumoral injections of AdCD40L were given every 7 days, followed by cytoreductive surgery in 9 cases and only immunotherapy in 10 cases. Tumor tissue was infiltrated with T and B lymphocytes after treatment, suggesting immune stimulation. The best overall response included 5 complete responses, 8 partial responses, and 4 stable and 2 progressive disease statuses according to the World Health Organization response criteria. Median survival was 160 days (range, 20-1141 d), with 3 dogs still alive at submission. Our results suggest that local AdCD40L therapy is safe and could have beneficial effects in dogs, supporting further treatment development. Clinical translation to human patients is in progress.Keywords
canine; melanoma; dogs; immunotherapy; AdCD40L; comparative oncologyPublished in
Journal of Immunotherapy2013, volume: 36, number: 6, pages: 350-358
Publisher: LIPPINCOTT WILLIAMS & WILKINS
Authors' information
Westberg, Sara (Saellström, Sara)
Swedish University of Agricultural Sciences, University Animal Hospital
Sadeghi, Arian
Svensson, Emma
Uppsala University
Segall, Thomas
Swedish University of Agricultural Sciences, University Animal Hospital
Korsgren, Olle
Hemminki, Akseli
University of Helsinki
Loskog, Angelica S. I.
Uppsala University
Tötterman, Thomas H.
Uppsala University
Von Euler, Henrik (Rönnberg, Henrik)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
UKÄ Subject classification
Cancer and Oncology
Publication Identifiers
DOI: https://doi.org/10.1097/CJI.0b013e31829d8a1b
URI (permanent link to this page)
https://res.slu.se/id/publ/54682