Skip to main content
SLU publication database (SLUpub)

Research article2013Peer reviewedOpen access

Loss in microbial diversity affects nitrogen cycling in soil

Philippot, Laurent; Spor, Ayme; Henault, Catherine; Bru, David; Bizouard, Florian; Jones, Christopher; Sarr, Amadou; Maron, Pierre-Alain

Abstract

Microbial communities have a central role in ecosystem processes by driving the Earth's biogeochemical cycles. However, the importance of microbial diversity for ecosystem functioning is still debated. Here, we experimentally manipulated the soil microbial community using a dilution approach to analyze the functional consequences of diversity loss. A trait-centered approach was embraced using the denitrifiers as model guild due to their role in nitrogen cycling, a major ecosystem service. How various diversity metrics related to richness, eveness and phylogenetic diversity of the soil denitrifier community were affected by the removal experiment was assessed by 454 sequencing. As expected, the diversity metrics indicated a decrease in diversity in the 1/10(3) and 1/10(5) dilution treatments compared with the undiluted one. However, the extent of dilution and the corresponding reduction in diversity were not commensurate, as a dilution of five orders of magnitude resulted in a 75% decrease in estimated richness. This reduction in denitrifier diversity resulted in a significantly lower potential denitrification activity in soil of up to 4-5 folds. Addition of wheat residues significantly increased differences in potential denitrification between diversity levels, indicating that the resource level can influence the shape of the microbial diversity-functioning relationship. This study shows that microbial diversity loss can alter terrestrial ecosystem processes, which suggests that the importance of functional redundancy in soil microbial communities has been overstated.

Keywords

biodiversity; ecosystem functioning; denitrification; functional redundancy; nitrogen cycling; soil

Published in

ISME Journal
2013, Volume: 7, number: 8, pages: 1609-1619
Publisher: NATURE PUBLISHING GROUP

    UKÄ Subject classification

    Microbiology
    Environmental Sciences
    Ecology

    Publication identifier

    DOI: https://doi.org/10.1038/ismej.2013.34

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/55519