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Research article2013Peer reviewedOpen access

Wax ester profiling of seed oil by nano-electrospray ionization tandem mass spectrometry

Even, Tim; Herrfurth, Cornelia; Heilmann, Mareike; Hofvander, Per; Stymne, Sten; Zhu, Li-Hua; Feussner, Ivo

Abstract

Conclusions: We developed a fast profiling method for wax ester analysis on the molecular species level. This method is suitable to screen large numbers of transgenic plants as well as other wax ester samples like cuticular lipid extracts to gain an overview on the molecular species composition. We confirm previous results from APCI-MS and GC-MS analysis, which showed that fragmentation patterns are highly dependent on the double bond distribution between the fatty alcohol and the fatty acid part of the wax ester.

Keywords

Jojoba seed oil; Lipid profiling; Wax ester molecular species

Published in

Plant Methods
2013, Volume: 9
Publisher: BIOMED CENTRAL LTD