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Research article - Peer-reviewed, 2005

Amino acid supplementation, controlled oxygen limitation and sequential double induction improves heterologous xylanase production by Pichia stipitis

Gorgens JF, Passoth V, van Zyl WH, Knoetze JH, Hahn-Hagerdal M

Abstract

Heterologous endo-beta-1,4-xylanase was produced by Pichia stipitis under control of the hypoxia-inducible PsADH2-promoter in a high-cell-clensity culture. After promoter induction by a shift to oxygen limitation, different aeration rates (oxygen transfer rates) were applied while maintaining oxygen-limitation. Initially, enzyme production was higher in oxygen-limited cultures with high rates of oxygen transfer, although the maximum xylanase activity was not significantly influenced. Amino acid supplementation increased the production of the heterologous endo-beta-1,4-xylanase significantly in highly aerated oxygen-limited cultures, until glucose was depleted. A slight second induction of the promoter was observed in all cultures after the glucose had been consumed. The second induction was most obvious in amino acid-supplemented cultures with higher oxygen transfer rates during oxygen limitation. When such oxygen-limited cultures were shifted back to fully aerobic conditions, a significant re-induction of heterologous endo-beta-1,4-xylanase production was observed. Re-induction was accompanied by ethanol consumption. A similar protein production pattern was observed when cultures were first grown on ethanol as sole carbon source and subsequently glucose and oxygen limitation were applied. Thus, we present the first expression system in yeast with a sequential double-inducible promoter. (c) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved

Published in

FEMS Yeast Research
2005, Volume: 5, number: 6-7, pages: 677-683
Publisher: ELSEVIER SCIENCE BV

    UKÄ Subject classification

    Agricultural Science
    Food Science

    Publication identifier

    DOI: https://doi.org/10.1016/j.femsyr.2004.12.003

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/5616