Skip to main content
Research article - Peer-reviewed, 2014

Chromogranins can be measured in samples from cats and dogs

Stridsberg, Mats; Pettersson, Ann; Hagman, Ragnvi; Westin, Christoffer; Höglund, Odd

Abstract

Background: Methods for objective evaluation of stress in animals are important, but clinically difficult. An alternative method to study the sympathetic activity may be to investigate Chromogranin A (CGA), Chromogranin B (CGB) and Secretogranin II (SG2). The aim of this study was to investigate the cross-reactivity of CGA, CGB and SG2 between man, cat and dog and to explore possibilities to measure these proteins in samples from cats and dogs. Results: Adrenal gland extracts from feline and canine species were measured by region-specific radioimmunoassays in different dilution steps to explore possible inter species cross reactivity. High cross reactivity was found for cats in the CGA17-38, CGA324-337, CGA361-372, CGB and SG2 assays. High cross reactivity was found for dogs in the CGA17-38, CGA361-372, CGB and SN assays. The method measuring the intact CGA was not useful for measurements in cats and dogs. Conclusions: Region-specific assays measuring defined parts of CGA, CGB and SG2 can be used for measurements in samples from cats and dogs. These results are promising and will allow for further studies of these proteins as possible clinical biomarkers in cats and dogs.

Keywords: Chromogranin, Secretogranin, Canine, Feline, Human, Radioimmunoassay

Keywords

Chromogranin; Secretogranin; Canine; Feline; Human; Radioimmunoassay

Published in

BMC Research Notes
2014, volume: 7, article number: 336

Authors' information

Stridsberg, Mats
Uppsala University
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Westin, Christoffer
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences

UKÄ Subject classification

Medicinal Chemistry
Physiology
Clinical Science
Endocrinology and Diabetes

Publication Identifiers

DOI: https://doi.org/10.1186/1756-0500-7-336

URI (permanent link to this page)

https://res.slu.se/id/publ/56288