Abstract

A novel Arabidopsis phospholipase A was identified. It existed in the cytosolic fraction, was not affected by Ca2+. It exhibited a strong headgroup specificity for phosphatidylcholine. The expression of AtLCAT-PLA inhibited yeast fatty acid accumulation. Its transcript in Arabidopsis was at high levels in roots and siliques. A previously uncharacterized Arabidopsis lecithin:cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine > phosphatidic acid > phosphatidylethanolamine > phosphatidylglycerol > phosphatidylserine and has an acyl chain specificity for oleoyl > linoleoyl > ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques

Keywords

Hydrolase; Phospholipase A; Arabidopsis thaliana; Saccharomyces cerevisiae

Published in

FEBS Letters
2012, Volume: 586, number: 4, pages: 373-377
Publisher: ELSEVIER SCIENCE BV

Conference

SLU Authors

• Lager, Ida

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Carlsson, Anders

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Stymne, Sten

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science
Renewable Bioenergy Research
Agricultural Science


Publication identifier

DOI: https://doi.org/10.1016/j.febslet.2011.12.034

Permanent link to this page (URI)

https://res.slu.se/id/publ/56308