Research article - Peer-reviewed, 2005
Nutrient effects on biocontrol of Penicillium roqueforti by Pichia anomala J121 during airtight storage of wheat
Druvefors UA, Passoth V, Schnurer JAbstract
The biocontrol yeast Pichia anomala inhibits the growth of a variety of mold species. We examined the mechanism underlying the inhibition of the grain spoilage mold Penicillium roqueforti by the biocontrol yeast P. anomala J121 during airtight storage. The biocontrol effect in a model grain silo with moist wheat (water activity of 0.96) was enhanced when complex medium, maltose, or glucose was added. Supplementation with additional nitrogen or vitamin sources did not affect the biocontrol activity of the yeast. The addition of complex medium or glucose did not significantly influence the yeast cell numbers in the silos, whether in the presence or absence of P. roqueforti. Mold growth was not influenced by the addition of nutrients, if cultivated without yeast. The products of glucose metabolism, mainly ethanol and ethyl acetate, increased after glucose addition to P. anomala-inoculated treatments. Our results suggest that neither competition for nutrients nor production of a glucose-repressible cell wall lytic enzyme is the main mode of action of biocontrol by P. anomala in this grain system. Instead, the mold-inhibiting effect probably is due to the antifungal action of metabolites, most likely a combination of ethyl acetate and ethanol, derived from glycolysis. The discovery that sugar amendments enhance the biocontrol effect of P. anomala suggests novel ways of formulating biocontrol yeastsPublished in
Applied and Environmental Microbiology2005, volume: 71, number: 4, pages: 1865-1869
Publisher: AMER SOC MICROBIOLOGY
Authors' information
Swedish University of Agricultural Sciences, Department of Microbiology
Swedish University of Agricultural Sciences, Department of Microbiology
Druvefors, Ulrika Ädel
Swedish University of Agricultural Sciences, Department of Microbiology
UKÄ Subject classification
Food Science
Agricultural Science
Publication Identifiers
DOI: https://doi.org/10.1128/AEM.71.4.1865-1869.2005
URI (permanent link to this page)
https://res.slu.se/id/publ/5640