The genetic dissection of immune response using gene-expression studies and genome mapping

Abstract

Functional genomics has been applied to the genetic dissection of immune response in different ways: (1) experimental crosses between lines that differ in their (non-) specific immune response have been used to detect quantitative trait loci (QTL) underlying these differences. (2) The measurement of gene expression levels for thousands of genes using microarrays or oligonucleotide chips to identify differential expression with regard to antigen challenge: (a) before and after infection, (b) resistant versus susceptible lines, or (c) combinations of both. Interpretation of QTL results is hampered by the fact that confidence regions of the QTL are large and can contain hundreds of potential candidate genes for the QTL. At the same time, the microarray experiments tend to show large numbers of differentially expressed genes without identifying the relationships between these genes. In the recently proposed 'genetical genomics' framework, members of a segregating population are characterised for genome-wide molecular markers and for gene expression levels. This facilitates the mapping of expression-QTL (eQTL): loci in the genome that control the expression of genes. Initial applications of this approach are critically reviewed and potential applications of this approach with regard to immune response are presented. (c) 2005 Elsevier B.V. All rights reserved.

Keywords

gene expression; genetics; immune response; mapping; quantitative trait locus

Published in

Veterinary Immunology and Immunopathology
2005, volume: 105, number: 3-4, pages: 343-352
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• de Koning, Dirk-Jan

  • The Roslin Institute

• Carlborg, Örjan

  • The Roslin Institute

UKÄ Subject classification

Genetics and Genomics

Publication identifier

• DOI: https://doi.org/10.1016/j.vetimm.2005.02.007

Permanent link to this page (URI)

https://res.slu.se/id/publ/57925