A generic real-time TaqMan assay for specific detection of lapinized Chinese vaccines against classical swine fever

Abstract

Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real-time assay that can detect all lapinized Chinese vaccines used widely, namely, Lapinized Philippines Coronel (LPC), Hog Cholera Lapinized virus (HCLV) and the Riems C-strain is still lacking. This study describes a real-time RT-PCR assay, targeting the N-pro gene region, for specific detection of these lapinized vaccine strains. The assay is highly sensitive, with a detection limit of 10 genome copies per reaction for HCLV and Riems C-strain and highly specific, as more than 100 strains of wild type CSFV representing all major genotypes were not detected. The assay is also highly repeatable: the coefficient of variation of Ct values in three runs was 2.77% for the detection of 10 copies of the vaccine viral RNA. This study provides a potentially useful tool for specific detection of the lapinized Chinese vaccines, HCLV and C-strain, and the differentiation of these vaccines from wild type CSFV. (C) 2011 Elsevier B.V. All rights reserved.

Keywords

CSFV; C-strain; Diagnosis; Lapinized Chinese vaccine

Published in

Journal of Virological Methods
2011, Volume: 175, number: 2, pages: 170-174 Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Xia, Hongyan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Belak, Sandor

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science


Publication identifier

DOI: https://doi.org/10.1016/j.jviromet.2011.05.003

Permanent link to this page (URI)

https://res.slu.se/id/publ/59208