Metreveli, Giorgi
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Research article2011Peer reviewed
Metreveli, Giorgi; Emmoth, Eva; Zohari, Siamak; Bálint, Ádám; Widén, Frederik; Muradrasoli, Shaman; Wallgren, Per; Belák, Sándor; LeBlanc, Neil; Berg, Mikael; Kiss, István; Widén, F
The influenza A virus subtypes H1N1, H1N2 and H3N2 are prevalent in pig populations worldwide. In the present study, two relatively uncommon swine influenza virus (SIV) H1N2 subtypes, isolated in Sweden in 2009 and 2010, were compared regarding their molecular composition and biological characteristics. The differences regarding markers purportedly related to pathogenicity, host adaptation or replication efficiency. They included a truncated PB1-F2 protein in the earlier isolate but a full length version in the more recent one; differences in the number of haemagglutinin glycosylation sites, including a characteristic human one; and a nuclear export protein with altered export signal. Of particular interest, the NS1 amino acid sequence of swine H1N2-2009 and 2010 has a 'unique or very unusual' PDZ binding domain (RPKV) at the C-terminal of the protein, a motif that has been implicated as a virulence marker. Concerning biological properties, these viruses reached lower titre and showed reduced cytopathogenicity in MDCK cells compared with an avian-like H1N1 isolate A/swine/Lidkoping/1193/2002 belonging to the same lineage as the 2009 and 2010 isolates. The findings should contribute to better understanding of factors related to the survival/extinction of this uncommon reassortant variant.
Influenza A; Swine influenza virus SIV; Genetic comparison
Virus Genes
2011, Volume: 42, number: 2, pages: 236-244 Publisher: SPRINGER
Veterinary Science
Genetics
Animal and Dairy Science
DOI: https://doi.org/10.1007/s11262-011-0571-2
https://res.slu.se/id/publ/59252