Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Research article2011Peer reviewed
Sperm selection using single layer centrifugation prior to cryopreservation can increase thawed sperm quality in stallions
Hoogewijs, Maarten; Morrell, Jane; van Soom, Ann; Govaere, Jan; Johannisson, Anders; Piepers, Sofie; De Schauwer, Catharina; de Kruif, Aart; De Vliegher, Sarne
Abstract
Reasons for performing study: The increasing use of modern reproductive techniques in human medicine has led to a higher demand for isolation of motile sperm. Several of these isolation techniques have been adapted for veterinary use and can be applied for the selection of a superior sperm sample from stallion semen. Until recently a major disadvantage of such isolation techniques was the limitation in sperm volume that could be handled. Androcoll-E had been shown to be successful for processing large volumes of equine semen but there are few data to substantiate the potential beneficial effect of freezing an Androcoll-E selected equine sperm sample to obtain higher quality following thawing.Objectives and methods: In this study, the effect of Androcoll-E treatment of sperm prior to cryopreservation was compared with cushioned centrifugation using ejaculates from 8 different stallions selected because they were known to have semen of differing quality following freezing.Results: Androcoll-E treatment increased measures of semen quality prior to freezing. However, Androcoll-E treatment reduced the yield of sperm following centrifugation when compared with the cushion centrifuged control group (50.9 +/- 14.2% vs. 97.1 +/- 9.0%, respectively). Quality analysis following thawing showed an overall improved sperm quality for Androcoll-E treated samples and average post thaw progressive motility (PM) was 41.6% compared with 30.5% for the cushion centrifuged group.Conclusions and potential relevance: Androcoll-E can be used with good results to select a superior sperm population prior to cryopreservation, in order to produce good-quality frozen thawed semen.
Keywords
horse; Androcoll-E; cryopreservation; equine semen; single layer centrifugation
Published in
Equine Veterinary Journal
2011, volume: 43, pages: 35-41
Publisher: WILEY-BLACKWELL
SLU Authors
Johannisson, Anders
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1111/j.2042-3306.2011.00489.x
Permanent link to this page (URI)
https://res.slu.se/id/publ/59458