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Research article2010Peer reviewed

Novel means of viral antigen identification: Improved detection of avian influenza viruses by proximity ligation

Schlingemann, Joerg; Leijon, Mikael; Yacoub, Alia; Schlingemann, Heidi; Zohari, Siamak; Matyi-Tóth, Anna; Kiss, István; Holmquist, Göran; Nordengrahn, Ann; Landegren, Ulf; Ekström, Björn; Belák, Sándor

Abstract

Recent outbreaks of avian influenza in different parts of the world have caused major economic losses for the poultry industry, affected wildlife seriously and present a significant threat even to human public health, due to the risk for zoonotic transmission. The ability to recognize avian influenza viruses (AIVs) early is of paramount importance to ensure that appropriate measures can be taken quickly to contain the outbreak. In this study. the performance of a proximity ligation assay (PLA) for the detection of AIV antigens in biological specimens was evaluated. It is shown that PLA: (i) as a novel principle of highly sensitive antigen detection is extending the arsenal of tools for the diagnosis of AIV; (ii) is very specific, nearly as sensitive as a commonly used reference real-time PCR assay, and four orders of magnitude more sensitive than a sandwich ELISA, utilizing the same antibody; (iii) avoids the necessity of nucleic acids extraction, which greatly facilitates high-throughput implementations; (iv) allows the use of inactivated samples, which safety can be transported from the field to diagnostic laboratories for further analysis. In summary, the results demonstrate that PLA is suited for rapid, accurate and early detection of AIV. (C) 2009 Elsevier B.V. All rights reserved.

Keywords

Infectious disease diagnosis; Proximity ligation; PCR; Monoclonal antibodies; Biotinylation; Avian influenza

Published in

Journal of Virological Methods
2010, volume: 163, number: 1, pages: 116-122
Publisher: ELSEVIER SCIENCE BV

SLU Authors

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science

Publication identifier

  • DOI: https://doi.org/10.1016/j.jviromet.2009.09.008

Permanent link to this page (URI)

https://res.slu.se/id/publ/59514