Knight, Stefan David
- Department of Molecular Biology, Swedish University of Agricultural Sciences
Research article2010Peer reviewedOpen access
Lindhorst, Thisbe; Märten, Michaela; Fuchs, Andreas; Knight, Stefan David
Mannose-specific adhesion of Escherichia coli bacteria to cell surfaces, the cause of various infections, is mediated by a fimbrial lectin, called FimH. X-ray studies have revealed a carbohydrate recognition domain (CRD) on FimH that can complex alpha-D-mannosides. However, as the precise nature of the ligand-receptor interactions in mannose-specific adhesion is not yet fully understood, it is of interest to identify carbohydrate recognition domains on the fimbrial lectin also in solution. Photoaffinity labeling serves as an appropriate methodology in this endeavour and hence biotin-labeled photoactive mannosides were designed and synthesized for photoaffinity labeling of FimH. So far, the photo-crosslinking properties of the new photoactive mannosides could be detailed with the peptide angiotensin II and labeling of FimH was shown both by MS/MS studies and by affino dot-blot analysis.
diazirines; FimH; lectins; MS/MS analysis; photoactive mannoside ligands; photoaffinity labeling
Beilstein Journal of Organic Chemistry
2010, Volume: 6, pages: 810-822
Publisher: BEILSTEIN-INSTITUT
Organic Chemistry
DOI: https://doi.org/10.3762/bjoc.6.91
https://res.slu.se/id/publ/59559