Kenne, Lennart
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-> 4-alpha-D-Glcp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-[alpha-D-Glcp-(1 -> 4)]-alpha-D-GlcpNAc-(1 -> 3)-alpha-L-FucpNAc-(1 -> 4)-alpha-D-Glcp-(1 -> P}(n). (C) 2009 Elsevier Ltd. All rights reserved.
Keywords
Hafnia alvei; O-antigen; Lipopolysaccharide; Phosphodiester; NMR spectroscopy; MALDI-TOF mass spectrometry
Published in
Carbohydrate Research
2010, volume: 345, number: 2, pages: 270-274
Publisher: ELSEVIER SCI LTD
SLU Authors
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.carres.2009.11.005
Permanent link to this page (URI)
https://res.slu.se/id/publ/59778