Abstract

The main aim of this study was to examine genotypic diversity of 82 Staphylococcus aureus isolates from cases of acute clinical mastitis in cattle using pulsed-field gel electrophoresis, and to investigate pulso-type differences between geographical regions of Sweden. The work also investigated haemolysis type (alpha, alpha beta), beta-lactamase production, and the association between S. aureus pulsotypes and breed, parity and presence of teat lesions. All isolates came from different farms.In total, 25 pulsotypes were found, but most only once or twice. The most common pulsotype accounted for 26% of the isolates and was found in all regions. The distribution of pulsotypes in the southern region of the country differed from the northern and middle regions. alpha beta- or alpha-Haemolysis was found in 66% and 34% of the isolates, respectively, and beta-lactamase production was found in 9% of the isolates. Variation in haemolysis type or beta-lactamase production was found within some pulsotypes. The results indicate that some pulsotypes have spread widely between herds within the country. (C) 2009 Elsevier Ltd. All rights reserved.

Keywords

Dairy cow; Clinical mastitis; Staphylococcus aureus; PFGE

Published in

Veterinary Journal
2010, Volume: 185, number: 2, pages: 188-192
Publisher: ELSEVIER SCI LTD

SLU Authors

• Persson Waller, Karin

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • National Veterinary Institute (SVA)

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science


Publication identifier

DOI: https://doi.org/10.1016/j.tvjl.2009.05.007

Permanent link to this page (URI)

https://res.slu.se/id/publ/60049