Abstract

In 2004, an atypical pestivirus named D32/00_'HoBi', isolated from foetal calf serum (FCS) originating from Brazil, was described (Schirrmeier et al., 2004). A few years later, a closely related virus (Th/04_KhonKaen) was detected in serum from a calf in Thailand, indicating that this group of atypical pestiviruses already is spread in cattle populations in various regions of the world. At the Friedrich-Loeffler-Institute, Insel Riems, Germany, FCS batches are regularly tested for pestivirus contamination, in general with positive PCR results, and in some cases the contaminants have been typed as 'HoBi'-like. At the National Veterinary Institute (SVA) in Uppsala, Sweden, a recent event with contaminated FCS ruined much of the ongoing cell culture work. From the FCS and the contaminated cells we were able to amplify and sequence nucleic acid from three different pestivirus strains, including BVDV-1, -2 and 'HoBi'-like; this in a commercial FCS that had been tested free from pestivirus by the manufacturer.In this short communication we review the current status of atypical 'HoBi'-like pestiviruses, describe recent findings and discuss the implications thereof. (C) 2009 Elsevier B.V. All rights reserved.

Keywords

Pestivirus; BVDV; HoBi; FCS; Contamination

Published in

Veterinary Microbiology
2010, Volume: 142, number: 1-2, pages: 90-93
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Ståhl, Karl

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • National Veterinary Institute (SVA)

• Belak, Sandor

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • National Veterinary Institute (SVA)

• Alenius, Stefan

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science


Publication identifier

DOI: https://doi.org/10.1016/j.vetmic.2009.09.048

Permanent link to this page (URI)

https://res.slu.se/id/publ/60172