Research article - Peer-reviewed, 2005
Effects of different cultivation temperatures on plasma membrane ATPase activity and lipid composition of sugar beet roots
Lindberg S, Banas A, Stymne SAbstract
Sugar beet seedlings (Beta vidgaris L. cv. Monohill) were cultivated for 3 weeks at different root and shoot temperatures and the plasma membranes (PM) from roots were purified by aqueous two-phase partitioning and analyzed for lipid composition and ATPase activities. Lipid analyses, undertaken immediately after PM purification from the roots, showed that a low root zone temperature (10 ° C) decreased the ratio between the major lipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A low temperature in the root environment increased the mol% of PE and decreased the mol% of phosphatidic acid (PA), independent on the shoot growth temperature. A low temperature also decreased the mol% of linoleic acid (18:2) and increased mol% of linolenic acid (18:3) in the analyzed lipid classes, especially ill PC and PE. The ratio between acyl chain lipids and protein generally increased in PM from roots grown at 10 ° C, compared with higher temperature. The changes in lipid composition correlated with changes in ATPase activities, detected as hydrolyses of MgATP. The kinetic parameters, K-m, and V of the PMH(+)ATPase in roots increased at a low cultivation temperature, independent on shoot temperature. Moreover, Arrhenius analyses showed that the transition temperature was independent of both root or shoot growth temperature at 10-24 ° C, whereas the activation energy of the ATPase was dependent oil the growth temperature of the root, and independent on shoot temperature. Thus, acclimation processes can take place in roots, irrespective of the shoot temperature. © 2005 Elsevier SAS. All rights reservedKeywords
Arrhenius analysis; H+ATPase; Lipids; Plasma membranes; Sugar beet; Temperature; UnsaturationPublished in
Plant Physiology and Biochemistry2005, volume: 43, number: 3, pages: 261-268
Publisher: EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Authors' information
Swedish University of Agricultural Sciences, Department of Crop Science
Lindberg, Sylvia
Banas, Antoni
UKÄ Subject classification
Agricultural Science
Food Science
Publication Identifiers
DOI: https://doi.org/10.1016/j.plaphy.2005.02.003
URI (permanent link to this page)
https://res.slu.se/id/publ/6018