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Research article - Peer-reviewed, 2010

The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp.

Plumridge, Andrew; Melin, Petter; Stratford, Malcolm; Novodvorska, Michaela; Shunburne, Lee; Dyer, Paul S; Roubos, Johannes A; Menke, Hildegard; Stark, Jacques; Stam, Hein; Archer, David B

Abstract

The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of the weak-acid preservative sorbic acid (2,4-hexadienoic acid) to 1,3-pentadiene. It is now shown that this decarboxylation process also requires the activity of a putative 4-hydroxybenzoic acid (3-octaprenyl-4-hydroxybenzoic acid) decarboxylase, encoded by a gene termed ohbA1, and a putative transcription factor, sorbic acid decarboxylase regulator, encoded by sdrA. The padA1, ohbA1 and sdrA genes are in close proximity to each other on chromosome 6 in the A. niger genome, and further bioinformatic analysis revealed conserved synteny at this locus in several Aspergillus species and other ascomycete fungi indicating clustering of metabolic function. This cluster is absent from the genomes of A. fumigatus and A. clavatus and, as a consequence, neither species is capable of decarboxylating sorbic acid. (C) 2010 Elsevier Inc. All rights reserved.

Keywords

Aspergillus; Saccharomyces; Sorbic acid; Decarboxylation; 1,3-Pentadiene; Cinnamic acid; Styrene; Gene regulation; Conidia; Germination

Published in

Fungal Genetics and Biology
2010, Volume: 47, number: 8, pages: 683-692
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

    UKÄ Subject classification

    Food Science

    Publication identifier

    DOI: https://doi.org/10.1016/j.fgb.2010.04.011

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/60325