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Research article2010Peer reviewed

Microdistribution of xyloglucan in differentiating Poplar cells

Sandquist, David; Filonova, Lada; von Schantz, Laura; Ohlin, Mats; Daniel, Geoffrey

Abstract

Recent studies on the ultrastructure and composition of the gelatinous layer (g-layer) in poplar have reported findings of xyloglucan. Using correlated fluorescence, scanning- and transmission electron microscopy, we found evidence for xyloglucan present in and surrounding the g-layer, using the fucosylated xyloglucan specific CCRC-M1 antibody and the carbohydrate binding module FXG-14b. However, labeling of isolated gelatinous layer remained negative.

Keywords

Populus tremula x tremuloides; Tension Wood; Xyloglucan; CBM; Immunogold labeling

Published in

Bioresources
2010, Volume: 5, number: 2, pages: 796-807
Publisher: NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI

      SLU Authors

    • Sandquist, David

      • Department of Forest Products, Swedish University of Agricultural Sciences

      • Stålhandske, Lada

        • Department of Forest Products, Swedish University of Agricultural Sciences

        • Daniel, Geoffrey

          • Department of Forest Products, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Bio Materials
        Wood Science

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/60588