Bankefors, Johan
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Research article2010Peer reviewed
Multidimensional profiling of components in complex mixtures of natural products for metabolic analysis, proof of concept: Application to Quillaja saponins
Bankefors, Johan; Nord, Lars; Kenne, Lennart
Abstract
A method for separation and detection of major and minor components in complex Mixtures has been developed, utilising two-dimensional high-performance liquid chromatography (2D-HPLC) combined with electrospray ionisation ion-trap multiple-stage mass spectrometry (ESI-ITMS(n)). Chromatographic conditions were matched with mass spectrometric detection to maximise the number of components that could be separated. The described procedure has proven useful to discern several hundreds of saponin components when applied to Quillaja saponaria Molina bark extracts. The discrimination of each saponin component relies oil the fact that three coordinates (x,y,z) for each component can be derived from the retention time of the two chromatographic steps (x,y) and the M/Z-values from the multiple-stage mass spectrometry (z(n), n = 1, 2....). Thus an improved graphical representation was obtained by combining retention times from the two-stage separation with +MS(1) (z(1)) and the additional structural information from the second mass stage +MS(2) (z(2), z(3)) corresponding to the main fragment ions. By this approach three-dimensional plots can be made that reveal both the chromatographic and structural properties of a specific mixture which can be useful in fingerprinting of complex mixtures. (C) 2009 Elsevier B.V. All rights reserved.
Keywords
Chromatographic profiling; Metabolic analysis; Multiple-stage mass spectrometry; Quillaja saponins; Two-dimensional chromatography
Published in
Journal of Chromatography B
2010, volume: 878, number: 3-4, pages: 471-476
Publisher: ELSEVIER SCIENCE BV
SLU Authors
Nord, Lars
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Kenne, Lennart
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Biochemistry and Molecular Biology
Biomedical Laboratory Science/Technology
Biochemistry
Molecular Biology
Publication identifier
- DOI: https://doi.org/10.1016/j.jchromb.2009.11.034
Permanent link to this page (URI)
https://res.slu.se/id/publ/60624