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Research article2014Peer reviewed

Adaptation of Dekkera bruxellensis to lignocellulose-based substrate

Tiukova, Ievgeniia; de Barros Pita, Will; Sundell, David; Haddad Momeni, Majid; Horn, Svein Jarle; Ståhlberg, Jerry; de Morais Jr, Marcos Antonio; Passoth, Volkmar

Abstract

Adaptation of Dekkera bruxellensis to lignocellulose hydrolysate was investigated. Cells of D. bruxellensis were grown for 72 and 192H in batch and continuous culture, respectively (adapted cells). Cultivations in semisynthetic medium were run as controls (nonadapted cells). To test the adaptation, cells from these cultures were reinoculated in the lignocellulose medium, and growth and ethanol production characteristics were monitored. Cells adapted to lignocellulose hydrolysate had a shorter lag phase, grew faster, and produced a higher ethanol concentration as compared with nonadapted cells. A stability test showed that after cultivation in rich medium, cells partially lost the adapted phenotype but still showed faster growth and higher ethanol production as compared with nonadapted cells. Because alcohol dehydrogenase genes have been described to be involved in the adaptation to furfural in Saccharomyces cerevisiae, an analogous mechanism of adaptation to lignocelluloses hydrolysate of D. bruxellensis was hypothesized. However, gene expression analysis showed that genes homologous to S. cerevisiae ADH1 were not involved in the adaptation to lignocelluloses hydrolysate in D. bruxellensis.

Keywords

Dekkera bruxellensis; lignocellulose; fermentation; adaptation

Published in

Biotechnology and Applied Biochemistry
2014, Volume: 61, number: SI 1, pages: 51-57 Publisher: WILEY-BLACKWELL

      SLU Authors

      • Haddad Momeni, Majid

        • Department of Molecular Biology, Swedish University of Agricultural Sciences
        • Ståhlberg, Jerry

          • Department of Molecular Biology, Swedish University of Agricultural Sciences
        • UKÄ Subject classification

          Microbiology

          Publication identifier

          DOI: https://doi.org/10.1002/bab.1145

          Permanent link to this page (URI)

          https://res.slu.se/id/publ/60942